The Gangreen Gang are the secondary, yet recurring, antagonists of "The Powerpuff Girls Movie". First, they appeared in the beginning, when the leader, Ace, punches the Professor before he goes home with groceries.
Later, they are the first villains the three girls, Blossom, Bubbles and Buttercup encounter. They are too scared to fight, not only because they are just little children, but also because they are forbidden to use their powers. However, they are hit by a lid, tossed at them by none other than Jojo.
Never again are they seen until the end of the movie, when as soon as the girls become Townsville's new, local superheroes, the Gang are stealing an old lady's groceries. This time, the Gang are scared of the girls, as Blossom punches out Ace, Bubbles punches out Snake and Grubber, and Buttercup punches out Big Billy and L'il Arturo. They are then put in jail with Mojo Jojo and Fuzzy Lumpkins.